Equipment
Flow Cytometers
FACSAria III
FACSAria III Sorter (acquired 2017)
Four lasers:
- 407 nm Solid State laser
- 488 nm Solid State laser
- 561 nm Solid State laser
- 633 nm Solid State laser
Fifteen parameter analysis:
- Forward Scatter
- Side Scatter
- 16 different fluorochromes
Sorting:
- separation into 1 – 4 populations
- single cell sorting into plates
FACSAria laser and filter configuration
LSRFortessa
BD LSRFortessa (acquired 2013)
Four lasers:
- 405 nm OBIS LX laser
- 488 Sapphire laser
- 561 Sapphire laser
- 628 OEM laser
Nineteen parameter analysis:
- Forward Scatter
- Side Scatter
- 17 different fluorochromes
User training is available
Fortessa Filter Config - 2023 Standard Configuration
Cytek Aurora
Cytek Aurora (acquired May 2021)
Three lasers:
- 405 nm Solid State violet
- 488 nm Solid State blue
- 640 nm Solid State red
Twenty-seven parameter analysis:
- Forward Scatter on blue laser
- Side Scatter on blue laser and violet laser
- 24 different fluorochromes
User training is available
Luminex Guava easyCyte HT System
Luminex Guava easyCyte HT System (acquired May 2021)
- 488 nm Solid State blue laser
- 96 well plate sample loader
5 parameters
- Forward Scatter
- Side Scatter
- 3 fluorescent
User training is available
Cell Separators
AutoMACS Sorter
AutoMACS Magnetic Sorter (acquired 2018) Magnetic bead based separation
- Quick, efficient single marker sorting based on cell surface phenotype
10x Genomics Chromium Controller
10x Genomics Chromium Controller (acquired June 2021)
- Single cell barcoding for 500 to 10,000 cells
- Gene Expression Analysis
- Immune Profiling
- ATAC
- Multiome ATAC + Gene Expression
- Spatial Gene Expression
10x Genomics Visium CytAssist
10x Genomics Visium CytAssist (acquired June 2023)
- Streamlines Visium workflow
- Spatial transcriptomics for gene expression
- Fresh-frozen tissue sections
- Fixed-frown tissue sections
- Formalin-fixed paraffin-embedded tissue sections
Other Instrumentation
Luminex MAGPIX Multiplexing System
The Luminex MAGPIX Multiplexing System was acquired in January 2024.
- Imaging based analyzer
- Measures up to 50 analytes in a single microplate well
- Read time of ≤60 minutes person 96-well plate
- Can provide up to 4,800 results per hour
User training is available.
Malvern NanoSight NS300
Malvern NanoSight NS300 (acquired 2017)
- Detects particles from 10nm to 2 micron in size
- Counts and sizes the particles
- Four lasers
- 405 nm diode laser
- 488 nm diode laser
- 532 nm diode laser
- 642 nm diode laser
- Four Filters
- 430 nm
- 500 nm
- 565 nm
- 650 nm
Nanosight NS300 laser and filter configuration
Malvern Zetasizer Nano Z
Malvern Zetasizer Nano Z (acquired 2017)
- Measures the zeta potential of particles in a solution
Miltenyi Biotec gentleMACS™ Otto Dissociator
Miltenyi Biotec gentleMACS™ Otto Dissociator (acquired 2015)
- Disassociates tissues into single cell suspension
- Homogenization of tissues or cells for downstream molecular biology applications
- Solid sample size of 20 mg – 4000 mg
- Liquid sample size of 300 ul – 10 ml
- 8 different samples can be run at one time
- Samples can be heated for enzymatic disassociation
Link to www.gentlemacs.com
MSD QuickPlex SQ120
MSD Multi-Array Imaging Platform (acquired 2019)
Electrochemiluminescence detection
Multiplexing Design:
- Cytokines
- Chemokines
- Cell Signaling Proteins
- Metabolic Proteins
- Available Assay Kits
Features:
- Detection Range 1-10,000 pg/ml
- Uses 25 ul or less of serum/plasma
- Detection with as little as 1 ug of cell extract
- Short assay time – approximately 4h
- Quick read time – approximately 3 min/plate