Equipment
Flow Cytometers
FACSAria III:
FACSAria III Sorter (acquired 2017)
Four lasers:
- 407 nm Solid State laser
- 488 nm Solid State laser
- 561 nm Solid State laser
- 633 nm Solid State laser
Fifteen parameter analysis:
- Forward Scatter
- Side Scatter
- 13 different fluorochromes
Sorting:
- separation into 2 – 4 populations
- sorting human primary cells and cell lines
FACSAria laser and filter configuration
LSRFortessa:
BD LSRFortessa (acquired 2013)
Four lasers:
- 405 nm OBIS LX laser
- 488 Sapphire laser
- 561 Sapphire laser
- 628 OEM laser
Fourteen parameter analysis:
- Forward Scatter
- Side Scatter
- 12 different fluorochromes
User training is available
Fortessa Laser Filter Config Blue 515 - Standard Configuration
Fortessa Optional Configuration B515-V585 on Standard Configuration
Cell Separators
Fluidigm C1 Single Cell Auto Prep System:
Fluidigm C1 Single Cell Auto Prep System (acquired 2015)
- 96 or 384 single cell format
- Isolates RNA from single cells and generate cDNA for downstream mRNAseq
- Isolate RNA, generates cDNA and pre-amplification for downstream PCR
- Isolate DNA for downstream DNAseq
Link to www.fluidigm.com/ifcs
AutoMACS Sorter:
AutoMACS Magnetic Sorter (acquired 2002) Magnetic bead based separation
- Quick, efficient single marker sorting based on cell surface phenotype
Other Instrumentation
Malvern NanoSight NS300:
Malvern NanoSight NS300 (acquired 2017)
- Detects particles from 10nm to 2 micron in size
- Counts and sizes the particles
- Four lasers
- 405 nm diode laser
- 488 nm diode laser
- 532 nm diode laser
- 642 nm diode laser
- Four Filters
- 430 nm
- 500 nm
- 565 nm
- 650 nm
Nanosight NS300 laser and filter configuration
Malvern Zetasizer Nano Z:
Malvern Zetasizer Nano Z (acquired 2017)
- Measures the zeta potential of particles in a solution
Miltenyi Biotec gentleMACS™ Otto Dissociator:
Miltenyi Biotec gentleMACS™ Otto Dissociator (acquired 2015)
- Disassociates tissues into single cell suspension
- Homogenization of tissues or cells for downstream molecular biology applications
- Solid sample size of 20 mg – 4000 mg
- Liquid sample size of 300 ul – 10 ml
- 8 different samples can be run at one time
- Samples can be heated for enzymatic disassociation
Link to www.gentlemacs.com
MSD Sector Imager 2400:
MSD Multi-Array Imaging Platform (acquired 2012)
Electrochemiluminescence detection
Multiplexing Design:
- Cytokines
- Chemokines
- Cell Signaling Proteins
- Metabolic Proteins
- Available Assay Kits
Features:
- Detection Range 1-10,000 pg/ml
- Uses 25 ul or less of serum/plasma
- Detection with as little as 1 ug of cell extract
- Short assay time – approximately 4h
- Quick read time – approximately 3 min/plate