West Virginia University Logo

Equipment


Flow Cytometers

FACSAria III

FACSAria III Sorter

FACSAria III Sorter (acquired 2017)
Four lasers:

  • 407 nm Solid State laser
  • 488 nm Solid State laser
  • 561 nm Solid State laser
  • 633 nm Solid State laser

Fifteen parameter analysis:

  • Forward Scatter
  • Side Scatter
  • 16 different fluorochromes

Sorting:

  • separation into 1 – 4 populations
  • single cell sorting into plates

FACSAria laser and filter configuration 


LSRFortessa

LSRFortessa

BD LSRFortessa (acquired 2013)

Four lasers:

  • 405 nm OBIS LX laser
  • 488 Sapphire laser
  • 561 Sapphire laser
  • 628 OEM laser

Nineteen parameter analysis:

  • Forward Scatter
  • Side Scatter
  • 17 different fluorochromes

User training is available

Fortessa Filter Config - 2023 Standard Configuration


Cytek Aurora

Cytek Aurora (acquired May 2021)

Three lasers:

  • 405 nm Solid State violet
  • 488 nm Solid State blue
  • 640 nm Solid State red

Twenty-seven parameter analysis:

  • Forward Scatter on blue laser
  • Side Scatter on blue laser and violet laser
  • 24 different fluorochromes

User training is available


Luminex Guava easyCyte HT System

Luminex Guava easyCyte HT System (acquired May 2021)

  • 488 nm Solid State blue laser
  • 96 well plate sample loader

5 parameters

  • Forward Scatter
  • Side Scatter
  • 3 fluorescent

User training is available


Cell Separators

AutoMACS Sorter

AutoMACS sorter

AutoMACS Magnetic Sorter (acquired 2018) Magnetic bead based separation

  • Quick, efficient single marker sorting based on cell surface phenotype

Miltenyibiotec.com

10x Genomics Chromium Controller

10x Genomics Chromium Controller (acquired June 2021)

  • Single cell barcoding for 500 to 10,000 cells
  • Gene Expression Analysis
  • Immune Profiling
  • ATAC
  • Multiome ATAC + Gene Expression
  • Spatial Gene Expression

10x Genomics Visium CytAssist

10x Genomics Visium CytAssist (acquired June 2023)

  • Streamlines Visium workflow
  • Spatial transcriptomics for gene expression
  • Fresh-frozen tissue sections
  • Fixed-frown tissue sections
  • Formalin-fixed paraffin-embedded tissue sections

Other Instrumentation

Malvern NanoSight NS300

Malvern NanoSight NS300

Malvern NanoSight NS300 (acquired 2017)

  • Detects particles from 10nm to 2 micron in size
  • Counts and sizes the particles
  • Four lasers
     - 405 nm diode laser
     - 488 nm diode laser
     - 532 nm diode laser
     - 642 nm diode laser
  • Four Filters
     - 430 nm
     - 500 nm
     - 
    565 nm
     - 650 nm

Nanosight NS300 laser and filter configuration


Malvern Zetasizer Nano Z

Malvern Zetasizer Nano Z

Malvern Zetasizer Nano Z (acquired 2017)

  • Measures the zeta potential of particles in a solution


Miltenyi Biotec gentleMACS™ Otto Dissociator

Miltenyi Biotec gentleMACS™ Otto Dissociator

 Miltenyi Biotec gentleMACS™ Otto Dissociator (acquired 2015)

  • Disassociates tissues into single cell suspension
  • Homogenization of tissues or cells for downstream molecular biology applications
  • Solid sample size of 20 mg – 4000 mg
  • Liquid sample size of 300 ul – 10 ml
  • 8 different samples can be run at one time
  • Samples can be heated for enzymatic disassociation 

Link to www.gentlemacs.com


MSD QuickPlex SQ120

MSD QuickPlex SQ120

MSD Multi-Array Imaging Platform (acquired 2019)
Electrochemiluminescence detection
Multiplexing Design:

Features:

  • Detection Range 1-10,000 pg/ml
  • Uses 25 ul or less of serum/plasma
  • Detection with as little as 1 ug of cell extract
  • Short assay time – approximately 4h
  • Quick read time – approximately 3 min/plate