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Equipment

 


Flow Cytometers




FACSAria III:

FACSAria III Sorter (acquired 2017)
Four lasers:

  • 407 nm Solid State laser
  • 488 nm Solid State laser
  • 561 nm Solid State laser
  • 633 nm Solid State laser


Fifteen parameter analysis:

  • Forward Scatter
  • Side Scatter
  • 13 different fluorochromes


Sorting:

  • separation into 2 – 4 populations
  • sorting human primary cells and cell lines


FACSAria laser and filter configuration 




LSRFortessa:

BD LSRFortessa (acquired 2013)

Four lasers:

  • 405 nm OBIS LX laser
  • 488 Sapphire laser
  • 561 Sapphire laser
  • 628 OEM laser

Fourteen parameter analysis:

  • Forward Scatter
  • Side Scatter
  • 12 different fluorochromes


User training is available

Fortessa Laser Filter Config Blue 515 - Standard Configuration
Fortessa Optional Configuration B515-V585 on Standard Configuration



Cell Separators




Fluidigm C1 Single Cell Auto Prep System:

Fluidigm C1 Single Cell Auto Prep System (acquired 2015)

  • 96 or 384 single cell format
  • Isolates RNA from single cells and generate cDNA for downstream mRNAseq
  • Isolate RNA, generates cDNA and pre-amplification for downstream PCR
  • Isolate DNA for downstream DNAseq


Link to www.fluidigm.com/ifcs




AutoMACS Sorter:

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AutoMACS Magnetic Sorter (acquired 2002) Magnetic bead based separation

  • Quick, efficient single marker sorting based on cell surface phenotype


Miltenyibiotec.com


Other Instrumentation




Malvern NanoSight NS300:

Malvern NanoSight NS300

Malvern NanoSight NS300 (acquired 2017)

  • Detects particles from 10nm to 2 micron in size
  • Counts and sizes the particles
  • Four lasers
     - 405 nm diode laser
     - 488 nm diode laser
     - 532 nm diode laser
     - 642 nm diode laser
  • Four Filters
     - 430 nm
     - 500 nm
     - 
    565 nm
     - 650 nm

Nanosight NS300 laser and filter configuration




Malvern Zetasizer Nano Z:

Malvern Zetasizer Nano Z

Malvern Zetasizer Nano Z (acquired 2017)

  • Measures the zeta potential of particles in a solution

Miltenyi Biotec gentleMACS™ Otto Dissociator:

 Miltenyi Biotec gentleMACS™ Otto Dissociator (acquired 2015)

  • Disassociates tissues into single cell suspension
  • Homogenization of tissues or cells for downstream molecular biology applications
  • Solid sample size of 20 mg – 4000 mg
  • Liquid sample size of 300 ul – 10 ml
  • 8 different samples can be run at one time
  • Samples can be heated for enzymatic disassociation 


Link to www.gentlemacs.com




MSD Sector Imager 2400:

MSD Sector Imager 2400

MSD Multi-Array Imaging Platform (acquired 2012)
Electrochemiluminescence detection
Multiplexing Design:

Features:

  • Detection Range 1-10,000 pg/ml
  • Uses 25 ul or less of serum/plasma
  • Detection with as little as 1 ug of cell extract
  • Short assay time – approximately 4h
  • Quick read time – approximately 3 min/plate